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axon genepix microarray scanner 4000b  (Danaher Inc)


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    Danaher Inc axon genepix microarray scanner 4000b
    Axon Genepix Microarray Scanner 4000b, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axon+genepix+4000b+microarray+scanner/GenePix+Microarray+Systems/pm22646476__nl2041707_si_001-194-1-6
    Average 94 stars, based on 460 article reviews
    axon genepix microarray scanner 4000b - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Microarray:

    Article Title: circ-BPTF serves as a miR-486-5p sponge to regulate CEMIP and promotes hypoxic pulmonary arterial smooth muscle cell proliferation in COPD.
    Article Snippet: Subsequently, the labelled cRNA was fragmented and hybridized onto the Arraystar human circRNA microarray (circular RNA array V2.0 RNA; Arraystar). .. The hybridized arrays were scanned using an Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, USA). .. Cell culture and proliferation assay As described previously [5–7], primary hPASMCs were separated from control subjects using distal pulmonary arteries and cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM; Biological Industries, Cromwell, USA) containing 10% fetal bovine serum (FBS; Biological Industries), 1% penicillin and streptomycin (Beyotime, shanghai, China) at 37°C.

    Article Title: Protective effect of endothelial progenitor cell-derived exosomal microRNA-382-3p on sepsis-induced organ damage and immune suppression in mice
    Article Snippet: Next, the amplified miRNA samples were labeled using a miRCURYTM Hy3/Hy5 Power kit (Exiqon, Vedbaek, Denmark) and hybridized using a miRCURYTM LNA Array kit (version 18.0, Exiqon). .. Then, the glass slides were scanned using an Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA, USA). .. The images were imported in the Genepix Pro (Version 6.0, Axon Instruments) for data extraction.

    Article Title: Herbal medicine Huazhuo Tiaozhi granule ameliorates dyslipidemia via regulating DDX17 lactylation and miR-155-5p biogenesis
    Article Snippet: Then the Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.18.0) (Exiqon, Vedbaek, Denmark) according to the array manual. .. The slides were scanned using an Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA). .. The scanned images were imported into GenePix Pro 6.0 software for grid alignment and data extraction.

    Article Title: Phosphorylation of MIF by PIP4K2a is necessary for cilia biogenesis
    Article Snippet: After Blocking with 5% bovine serum albumin for 1 h at room temperature, protein chips were incubated with purified human MIF protein (50 nM, R&D Systems) or 5% bovine serum albumin for 1 h. Protein interaction was then determined by sequentially incubating with either rabbit anti-MIF antibody and goat anti-rabbit immunoglobulin G (IgG)-Cy3-conjugated antibody or goat anti-rabbit immunoglobulin G (IgG)-Cy3-conjugated antibody (negative controls). .. Axon GenePix 4000B Microarray Scanner (Molecular Devices, LLC, Sunnyvale, CA), and the probe signals were acquired using GenePix Pro 6.0 software (Molecular Devices). ..

    Article Title: lncRNA TUG1 as a ceRNA promotes PM exposure-induced airway hyper-reactivity.
    Article Snippet: .. For microRNAs, after miRNA labeling and array hybridization, the slides were scanned utilizing the Axon GenePix 4000B microarray scanner (Axon Instruments, CA). .. Then, scanned images were analyzed by GenePix Pro 6.0 software (Axon Instruments, CA) to extract raw expression data.

    Article Title: The sulfur metabolism regulator MetR is a global regulator controlling phytochrome-dependent light responses in Aspergillus nidulans.
    Article Snippet: Phytochrome-dependent light signaling has been studied in several fungi.. In Aspergillus nidulans lightstimulated phytochrome activates the high-osmolarity glycerol (HOG) signaling pathway and thereby controls the expression of a large number of genes, many of which are related to stress responses.. In a genome-wide expression analysis in A. nidulans we found that phytochrome, fphA, is under strict expression control of the central regulator of the sulfur-starvation response, MetR.

    Article Title: The silent information regulator 1 pathway attenuates ROS-induced oxidative stress in Alzheimer's disease.
    Article Snippet: CircRNA and miRNAwere labeled using miRCURYTM Hy3TM/Hy5TM Power labeling kit (Exiqon, Vedbaek, Denmark) and were hybridized using the miRCURYTM LNA Array (v.18.0) (Exiqon, Vedbaek, Denmark). .. Slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA, USA). .. Total RNA was extracted using TRIzol reagent (Invitrogen, USA).

    Article Title: Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation
    Article Snippet: .. Specifically, reverse transcription was performed using the SuperScript Double-Stranded cDNA Synthesis kit (Thermo Fisher Scientific, Waltham, USA); ds-cDNA labeling was performed using the NimbleGen one-color DNA labeling kit (Roche Diagnostics, Mannheim, Germany); array hybridization was performed using the NimbleGen Hybridization System followed by washing with the NimbleGen wash buffer kit (Roche Diagnostics, 05584507001); and array scanning was performed using the Axon GenePix 4000B microarray scanner (Molecular Devices LLC, Sunnyvale, CA, USA). .. The raw signal intensities were extracted and normalized using the Robust Multichip Average (RMA) method with NimbleScan v2.5 software (Roche NimbleGen), and low intensity (<100.0) genes were filtered.

    Software:

    Article Title: Phosphorylation of MIF by PIP4K2a is necessary for cilia biogenesis
    Article Snippet: After Blocking with 5% bovine serum albumin for 1 h at room temperature, protein chips were incubated with purified human MIF protein (50 nM, R&D Systems) or 5% bovine serum albumin for 1 h. Protein interaction was then determined by sequentially incubating with either rabbit anti-MIF antibody and goat anti-rabbit immunoglobulin G (IgG)-Cy3-conjugated antibody or goat anti-rabbit immunoglobulin G (IgG)-Cy3-conjugated antibody (negative controls). .. Axon GenePix 4000B Microarray Scanner (Molecular Devices, LLC, Sunnyvale, CA), and the probe signals were acquired using GenePix Pro 6.0 software (Molecular Devices). ..

    Labeling:

    Article Title: lncRNA TUG1 as a ceRNA promotes PM exposure-induced airway hyper-reactivity.
    Article Snippet: .. For microRNAs, after miRNA labeling and array hybridization, the slides were scanned utilizing the Axon GenePix 4000B microarray scanner (Axon Instruments, CA). .. Then, scanned images were analyzed by GenePix Pro 6.0 software (Axon Instruments, CA) to extract raw expression data.

    Article Title: Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation
    Article Snippet: .. Specifically, reverse transcription was performed using the SuperScript Double-Stranded cDNA Synthesis kit (Thermo Fisher Scientific, Waltham, USA); ds-cDNA labeling was performed using the NimbleGen one-color DNA labeling kit (Roche Diagnostics, Mannheim, Germany); array hybridization was performed using the NimbleGen Hybridization System followed by washing with the NimbleGen wash buffer kit (Roche Diagnostics, 05584507001); and array scanning was performed using the Axon GenePix 4000B microarray scanner (Molecular Devices LLC, Sunnyvale, CA, USA). .. The raw signal intensities were extracted and normalized using the Robust Multichip Average (RMA) method with NimbleScan v2.5 software (Roche NimbleGen), and low intensity (<100.0) genes were filtered.

    Hybridization:

    Article Title: lncRNA TUG1 as a ceRNA promotes PM exposure-induced airway hyper-reactivity.
    Article Snippet: .. For microRNAs, after miRNA labeling and array hybridization, the slides were scanned utilizing the Axon GenePix 4000B microarray scanner (Axon Instruments, CA). .. Then, scanned images were analyzed by GenePix Pro 6.0 software (Axon Instruments, CA) to extract raw expression data.

    Article Title: The sulfur metabolism regulator MetR is a global regulator controlling phytochrome-dependent light responses in Aspergillus nidulans.
    Article Snippet: Phytochrome-dependent light signaling has been studied in several fungi.. In Aspergillus nidulans lightstimulated phytochrome activates the high-osmolarity glycerol (HOG) signaling pathway and thereby controls the expression of a large number of genes, many of which are related to stress responses.. In a genome-wide expression analysis in A. nidulans we found that phytochrome, fphA, is under strict expression control of the central regulator of the sulfur-starvation response, MetR.

    Article Title: Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation
    Article Snippet: .. Specifically, reverse transcription was performed using the SuperScript Double-Stranded cDNA Synthesis kit (Thermo Fisher Scientific, Waltham, USA); ds-cDNA labeling was performed using the NimbleGen one-color DNA labeling kit (Roche Diagnostics, Mannheim, Germany); array hybridization was performed using the NimbleGen Hybridization System followed by washing with the NimbleGen wash buffer kit (Roche Diagnostics, 05584507001); and array scanning was performed using the Axon GenePix 4000B microarray scanner (Molecular Devices LLC, Sunnyvale, CA, USA). .. The raw signal intensities were extracted and normalized using the Robust Multichip Average (RMA) method with NimbleScan v2.5 software (Roche NimbleGen), and low intensity (<100.0) genes were filtered.

    Reverse Transcription:

    Article Title: Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation
    Article Snippet: .. Specifically, reverse transcription was performed using the SuperScript Double-Stranded cDNA Synthesis kit (Thermo Fisher Scientific, Waltham, USA); ds-cDNA labeling was performed using the NimbleGen one-color DNA labeling kit (Roche Diagnostics, Mannheim, Germany); array hybridization was performed using the NimbleGen Hybridization System followed by washing with the NimbleGen wash buffer kit (Roche Diagnostics, 05584507001); and array scanning was performed using the Axon GenePix 4000B microarray scanner (Molecular Devices LLC, Sunnyvale, CA, USA). .. The raw signal intensities were extracted and normalized using the Robust Multichip Average (RMA) method with NimbleScan v2.5 software (Roche NimbleGen), and low intensity (<100.0) genes were filtered.

    cDNA Synthesis:

    Article Title: Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation
    Article Snippet: .. Specifically, reverse transcription was performed using the SuperScript Double-Stranded cDNA Synthesis kit (Thermo Fisher Scientific, Waltham, USA); ds-cDNA labeling was performed using the NimbleGen one-color DNA labeling kit (Roche Diagnostics, Mannheim, Germany); array hybridization was performed using the NimbleGen Hybridization System followed by washing with the NimbleGen wash buffer kit (Roche Diagnostics, 05584507001); and array scanning was performed using the Axon GenePix 4000B microarray scanner (Molecular Devices LLC, Sunnyvale, CA, USA). .. The raw signal intensities were extracted and normalized using the Robust Multichip Average (RMA) method with NimbleScan v2.5 software (Roche NimbleGen), and low intensity (<100.0) genes were filtered.

    DNA Labeling:

    Article Title: Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation
    Article Snippet: .. Specifically, reverse transcription was performed using the SuperScript Double-Stranded cDNA Synthesis kit (Thermo Fisher Scientific, Waltham, USA); ds-cDNA labeling was performed using the NimbleGen one-color DNA labeling kit (Roche Diagnostics, Mannheim, Germany); array hybridization was performed using the NimbleGen Hybridization System followed by washing with the NimbleGen wash buffer kit (Roche Diagnostics, 05584507001); and array scanning was performed using the Axon GenePix 4000B microarray scanner (Molecular Devices LLC, Sunnyvale, CA, USA). .. The raw signal intensities were extracted and normalized using the Robust Multichip Average (RMA) method with NimbleScan v2.5 software (Roche NimbleGen), and low intensity (<100.0) genes were filtered.



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